Journal: Clinical Epigenetics

Article Title: Quantitative methodology is critical for assessing DNA methylation and impacts on correlation with patient outcome

doi: 10.1186/1868-7083-6-22

Figure Lengend Snippet: Literature identified hypermethylated loci in head and neck squamous cell carcinomas, according to frequency, methodology and univariate correlation with survival a

Article Snippet: For the RUNX3 pyrosequencing analysis, 83 samples had sufficient bisulfite modified DNA available for assessment.

Techniques: Methylation

Journal: Clinical Epigenetics

Article Title: Quantitative methodology is critical for assessing DNA methylation and impacts on correlation with patient outcome

doi: 10.1186/1868-7083-6-22

Figure Lengend Snippet: Summary of methylation events for each locus assessed by methylation sensitive high resolution melting (MS-HRM), and assessed in the Cancer Genome Atlas (TCGA) cohort a

Article Snippet: For the RUNX3 pyrosequencing analysis, 83 samples had sufficient bisulfite modified DNA available for assessment.

Techniques: Methylation

Journal: Clinical Epigenetics

Article Title: Quantitative methodology is critical for assessing DNA methylation and impacts on correlation with patient outcome

doi: 10.1186/1868-7083-6-22

Figure Lengend Snippet: Pyrosequencing results for all samples demonstrating heterogeneous methylation of RUNX3 when assessed by methylation sensitive high resolution melting (MS-HRM). Each analyzed sample is annotated for the quantity of methylation detected at each CpG site interrogated, and the mean methylation value for all CpG sites analyzed is also provided. This figure demonstrates that the vast majority of samples with heterogeneous methylation for this locus assessed by MS-HRM, had low levels of methylation when quantified by pyrosequencing.

Article Snippet: For the RUNX3 pyrosequencing analysis, 83 samples had sufficient bisulfite modified DNA available for assessment.

Techniques: Methylation